Beetroot Extract as Fluorescence Probes for Copper Ion Detection in Aqueous Solution

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Published: Jun 28, 2025

Abstract:

Backgorund of Study: Copper contamination in water poses serious health and environmental risks. Excessive exposure can lead to critical human health issues and is toxic to aquatic organisms. Existing detection techniques are often expensive and complex, highlighting the need for more accessible, eco-friendly alternatives.


Aims and Scope of Paper: This study explores betanin from beetroot as a low-cost, eco-friendly fluorescence probe for detecting copper ions, highlighting its sensitivity and potential for environmental and biomedical use.


Methods: Beetroot extract was analyzed using UV-Vis, PL, and FTIR spectroscopy to examine changes in betanin’s optical properties in response to different copper ion concentrations.


Result: The fluorescence intensity of betanin decreased as copper ion concentration increased. A linear correlation (R² = 0.792) was observed between copper levels and absorbance, and the sensitivity was calculated at –6.21828 × 10⁻⁴ A.U./ppm, demonstrating betanin's strong potential as a copper ion detector.


Conclusion: The study confirms betanin from beetroot shows strong potential as a low-cost, eco-friendly copper ion detector, supporting sustainable sensing solutions across various fields.

Keywords: Aquepus Solution, Beetroot dye, Fourier Transform Infrared, Photoluminescence, Ultraviolet-visible

Authors:
1 . Ganesan Krishnan
1 . Nurul Badrina Aiman Rashidi
1 . Zakirah Buhari
How to Cite
Krishnan, G., Nurul Badrina Aiman Rashidi, & Zakirah Buhari. (2025). Beetroot Extract as Fluorescence Probes for Copper Ion Detection in Aqueous Solution. International Journal of Sustainable Engineering Innovations, 1(1), 6–10. Retrieved from https://e-journal.gomit.id/ijsei/article/view/3
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References

Borjan, D., Šeregelj, V., Andrejč, D. C., Pezo, L., Šaponjac, V. T., Knez, Ž., Vulić, J., & Marevci, M. K. (2022). Green Techniques for Preparation of Red Beetroot Extracts with Enhanced Biological Potential. Antioxidants, 11(5), 1–19. https://doi.org/10.3390/antiox11050805

Gomes, V., Mateus, N., de Freitas, V., & Cruz, L. (2021). A pH-responsive fluorescent sensor based on a new pyranoxanthylium salt. Photochemical and Photobiological Sciences, 20(4), 513–521. https://doi.org/10.1007/s43630-021-00033-x

Li, L., Wang, J., Xu, S., Li, C., & Dong, B. (2022). Recent Progress in Fluorescent Probes For Metal Ion Detection. Frontiers in Chemistry, 10(April), 1–15. https://doi.org/10.3389/fchem.2022.875241

Maillard, J., Klehs, K., Rumble, C., Vauthey, E., Heilemann, M., & Fürstenberg, A. (2021). Universal quenching of common fluorescent probes by water and alcohols. Chemical Science, 12(4), 1352–1362. https://doi.org/10.1039/d0sc05431c

Vettumperumal, R., Kalyanaraman, S., Selvan, G. T., & Selvakumar, P. M. (2018). Fluorescence analysis of natural dyes from Plumeria rubra (red and white) flowers. Optik, 159, 108–114. https://doi.org/10.1016/j.ijleo.2018.01.070

Zia, P., Sunita, M., & Sneha, S. (2021). Extraction of Natural Colour from Beet Root (Beta vulgaris) its Phytochemical Analysis and Antibacterial Activity. Original Research Article, 1873(4), 2663–7308. https://doi.org/10.36349/easjnfs.2021.v03i04.002